Services

The Ralph M. Parsons Plant Transformation Facility at UC Davis provides routine plant transformation services for the plant species listed in the table below for clients within the continental United States.

service

Please note that the Facility will no longer be transforming plasmids into Agrobacterium for client orders. Clients are required to provide the facility with engineered Agrobacterium cultures containing their gene of interest. For clients outside the state of California a USDA-APHIS permit is required for interstate shipments of genetically engineered Agrobacterium. Please visit the USDA-APHIS-Biotechnology website for additional information on the permitting process. California clients shipping genetically engineered Agrobacterium to the facility intrastate, should submit a courtesy letter to CDFA. Please contact the facility for additional information and assistance regarding the interstate or intrastate permitting process

Placing an Order

To place an border, click on our Order tab and log in to our interactive website. You can check the status of your order at any time by logging in to your account.

Crop Genotype Selection System # of
Independent
Events
UC Clients
Cost
Non-UC Clients Cost*
Alfalfa - Medicago sativa Regen kanamycin

10 $800 $1,670
Canola Westar kanamycin 5 $800 not available
Citrus Carrizo kanamycin 5 $800 $1,670
Lettuce - Lactuca sativa most genotypes kanamycin 10 $800 $1,670
Pepper R&C Cayenne glufosinate 5 $800 $1,670
Petunia Mitchell diploid kanamycin 10 $800 $1,670
Rice Kitaake hygromycin 10 $800 $1,670
G418 10 $800 $1,670
Tobacco Benthamiana

kanamycin
hygromycin
glufosinate

10 $800 $1,670
Tobacco - Nicotiana Sr1, Samsun, TI1347, Xanthi kanamycin
hygromycin
glufosinate
10
$400 $835
Tomato -
S. lycopersicum
,
S. habrochaites,
S. pimpinellifolium,

Money maker, T-5, UC 82B, VF 36, MicroTom, etc**
please note, if requesting kanamycin selection
for tomato, the nptii gene must be driven by the nos
or mas promoter, not by 35s. for glufosinate the bar

must be driven by the 35s promoter
Please note- we can supply seeds of Moneymaker,

T5 and Microtom, but for all other cultivars, the client

needs to provide 200 seeds per construct

kanamycin
hygromycin
glufosinate

10 $800 $1,670
Wheat*** Fielder hygromycin 5 $1,000 $2,000

* The higher price for non-UC clients reflects non-University differential (NUD) charges
** Many other cultivars can be transformed, including a wide range of wild tomato relatives-contact the facility for details

*** Certain restrictions apply- please contact the facility for details.

Protocol Development

The Facility's staff has extensive experience in the development of transformation protocols for species for which transformation methodologies are not available or are currently inefficient. The facility also has significant expertise in protocol development for a wide range of tissue culture processes including micropropagation, plant regeneration, tetraploidization, protoplast isolation etc. Please contact the facility for information on protocol development or optimization services for plant tissue culture or transformation protocols.

Minimum Fee

We make every effort to successfully produce and deliver the number of plants requested by clients using our transformation service. However, on occasion there are extenuating circumstances that prevent us from completing an order. These include; defective or contaminated Agrobacterium cultures, constructs that inadvertently lack a selectable marker gene, constructs that contain genes of interest that are lethal to plant tissue, and constructs that contain genes that act as phytohormones thereby interfering with the normal plant regeneration process. Since the nature of our work requires that we invest a significant amount of up front effort prior to identifying these types of problems, the customer is required to pay 20% of the total recharge fee even if we are unsuccessful in regenerating the requested number of plants.

Molecular Analysis

We do not analyze the transgenic plants for your gene of interest, but we do run a bioassay to confirm the presence of the selectable marker gene.